00551nas a2200157   4500008003900000245006700039210006600106260001800172653001900190100001500209700001600224700001700240700001400257700001800271856010400289       2013                          d00aTowards a controlled and monitorable hepatic micro-environment0 aTowards a controlled and monitorable hepatic microenvironment  aLiverpool, UK10aBioengineering1 aTirella, A1 aLa Marca, M1 aCarroll, L A1 aAylott, J1 aAhluwalia, A.  uhttp://95.110.227.16/publications/towards-controlled-and-monitorable-hepatic-micro-environment.html01701nas a2200157   4500008003900000245009400039210006900133260001600202520112500218653001901343100001501362700001701377700001401394700001801408856011701426       2012                          d00aMonitoring cell culture 3D microenvironments: oxygen and pH nano-sensors within hydrogels0 aMonitoring cell culture 3D microenvironments oxygen and pH nanos  aRome, Italy3 a<p>Ratiometric pH and O2 nanosensors were fabricated independently using a fluorophore that produces a signal proportional to the concentration of the analyte of interest, and a second fluorophore that produces a reference signal, insensitive to the analyte of interest. These fluorophores emit at different wavelengths and were either entrapped or covalently bound, within an inert optically transparent polymeric matrix. In this work uniform sized 3D micro-scaled alginate hydrogel constructs of approximately 100-300 $μ$m were fabricated. Through dual incorporation of HepG2 cells and nanosensors within these alginate constructs we aim to have a real-time, non-invasive method to measure microenvironmental pH and O2 content. Ratiometric fluorescent output from the microenvironment is used to monitor O2 concentrations and pH during cell culture. Measurements show that 3D micro-scaled constructs are suitable for cell growth and proliferation. Moreover O2 and pH values within the hydrogel cellularised microspheres are shown to have physiological values that enable the maintenance of the hepatic phenotype</p>
10aBioengineering1 aTirella, A1 aCarroll, L A1 aAylott, J1 aAhluwalia, A.  uhttp://95.110.227.16/publications/monitoring-cell-culture-3d-microenvironments-oxygen-and-ph-nano-sensors-within02004nas a2200169   4500008003900000245009200039210006900131260002000200520139200220653001901612653001401631100001501645700001801660700001401678700001701692856012501709       2012                          d00a{Monitoring microconstructs: cell and nanosensor encapsulation in alginate micro-beads}0 aMonitoring microconstructs cell and nanosensor encapsulation in   aVienna, Austria3 a<p>Monitoring and controlling the microenvironment of cell cultures is an ongoing challenge for many researchers. Much research has been conducted characterising individual aspects such as 3D architecture, mechanical properties, biochemicals, etc. The biggest deficits in existing models for monitoring analytes within the cellular environment is the lack of appropriate means for non invasive, real-time and integrated monitoring of the cellular responses. Nanosensors can overcome these issues: they are porous polymeric nanoparticles that are sensitive to a range of analytes including pH and O2. Microfabrication techniques are innovative tools to obtain controlled microstructures with a defined 3D architecture. In this work uniform sized 3D micro-scaled hydrogel constructs of approximately 300-400 um diameter were fabricated. Through dual incorporation of cells and nanosensors within these constructs we aim to have a real-time, non invasive method to measure microenvironmental pH value and O2 content. Ratiometric fluorescent output from the microenvironment is used to monitor O2 and pH during cell culture. Measurements show that 3D micro-scaled constructs are suitable for cell growth and proliferation. Moreover O2 content and pH within hydrogel cellularised microspheres are shown to have physiological values which enable the maintenance of the hepatic phenotype.</p>
10aBioengineering10aNanotools1 aTirella, A1 aAhluwalia, A.1 aAylott, J1 aCarroll, L A  uhttp://95.110.227.16/publications/monitoring-microconstructs-cell-and-nanosensor-encapsulation-alginate-micro-beads.html