00574nas a2200133   4500008004100000245013200041210006900173300001300242653001900255100001500274700001500289700001800304856011800322       2012                        eng d00aFunctionally Graded Materials (FGMs) with predictable and controlled gradient profiles: computational modelling and realisation0 aFunctionally Graded Materials FGMs with predictable and controll  aaccepted10aBioengineering1 aMattei, G.1 aTirella, A1 aAhluwalia, A.  uhttp://95.110.227.16/publications/functionally-graded-materials-fgms-predictable-and-controlled-gradient-profiles02003nas a2200193   4500008004100000022001400041245015000055210006900205300001600274490000700290520137000297653001901667100001401686700001501700700001501715700001301730700001801743856004801761       2010                        eng d  a1879-317700aFinite element modelling and design of a concentration gradient generating bioreactor: Application to biological pattern formation and toxicology0 aFinite element modelling and design of a concentration gradient   a1828–18370 v243 a<p>This paper describes the use of a microfluidic gradient maker for the toxicological analysis of some conventional biomolecules such as hydrogen peroxide and a local anaesthetic, lidocaine on different cell cultures, human endothelial cells and myoblasts, respectively. The microfluidic device was designed and simulated using COMSOL Multiphysics(R) and the concentration gradient in the microfluidic network was analysed through a fluid-dynamic and mass-transport study. Subsequently the device was fabricated with soft lithography, casting PDMS in a master to obtain channels about 250mum deep. Hydrogen peroxide was tested on human endothelial cells, while lidocaine was tested on C2C12 myoblasts and an analysis was performed using propidium iodide staining followed by an imaging processing routine to obtain quantitative dose-response profiles in the gradient maker. The results show that the Gradient Maker (GM) bioreactor is a more sensitive method for detection of cell toxicity, and compared with testing of drug toxicity using microwells with individual cell cultures, allows one shot testing with a single cell culture exposed to a large number of concentrations. Moreover, the Gradient Maker was also modelled in order to realise biological pattern formation using two morphogenes acting as activator and inhibitor with varying diffusion rates.</p>
10aBioengineering1 aVozzi, G.1 aMazzei, D.1 aTirella, A1 aVozzi, F1 aAhluwalia, A.  uhttp://www.ncbi.nlm.nih.gov/pubmed/20580814