01787nas a2200205   4500008004100000022001400041245013800055210006900193520112600262653001901388100001701407700001701424700001401441700001501455700001301470700001701483700001501500700001801515856004801533       2013                        eng d  a1743-540400aIn vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response0 aIn vitro toxicological screening of nanoparticles on primary hum3 a<p>Abstract After passage through biological barriers, nanomaterials inevitably end up in contact with the vascular endothelium and physiological flow, and can induce cardiovascular damage. In this study the toxicity and sublethal effects of 6 nanoparticles, including 4 of industrial and biomedical importance, on human endothelial cells was investigated using different in vitro assays. The results show that all the particles investigated induce some level of damage to the cells and that silver particles were most toxic, followed by titanium dioxide. Furthermore endothelial cells were shown to be more susceptible when exposed to silver nanoparticles under flow conditions in a bioreactor. The study underlines that although simple in vitro tests are useful to screen compounds and to identify the type of effect induced on cells, they may not be sufficient to define safe exposure limits. Therefore, once initial toxicity screening has been conducted on nanomaterials, it is necessary to develop more physiologically relevant in vitro models to better understand how nanomaterials can impact on human health.</p>
10aBioengineering1 aUcciferri, N1 aCollnot, E M1 aGaiser, B1 aTirella, A1 aStone, V1 aDomenici, C.1 aLehr, C -M1 aAhluwalia, A.  uhttp://www.ncbi.nlm.nih.gov/pubmed/2390970301696nas a2200217   4500008004100000245008200041210006900123260000800192300001400200490000600214520101700220653001901237100001301256700001701269700001401286700001501300700001701315700001601332700001801348856011201366       2010                        eng d00aIn vitro liver model using microfabricated scaffolds in a modular bioreactor.0 aIn vitro liver model using microfabricated scaffolds in a modula  cFeb  a232–2410 v53 a<p>Hepatocyte function on 3-D microfabricated polymer scaffolds realised with the pressure-activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose consumption, and albumin secretion rate were measured daily over a week. Cells seeded on scaffolds showed an increase in cell density compared with monolayer controls. Moreover, in dynamic culture, cell metabolic function increased three times in comparison with static monolayer cultures. These results suggest that cell density and cell-cell interactions are mediated by the architecture of the substrate, while the endogenous biochemical functions are regulated by a sustainable supply of nutrients and interstitial-like flow. Thus, a combination of 3-D scaffolds and dynamic flow conditions are both important for the development of a hepatic tissue model for applications in drug testing and regenerative medicine.</p>
10aBioengineering1 aVinci, B1 aCavallone, D1 aVozzi, G.1 aMazzei, D.1 aDomenici, C.1 aBrunetto, M1 aAhluwalia, A.  uhttp://95.110.227.16/publications/vitro-liver-model-using-microfabricated-scaffolds-modular-bioreactor.html