01494nas a2200181   4500008004100000022001400041245008800055210006900143300001100212490000600223520095500229653001901184100001501203700001401218700001401232700001801246856004801264       2009                        eng d  a1758-509000aA phase diagram for microfabrication of geometrically controlled hydrogel scaffolds0 aphase diagram for microfabrication of geometrically controlled h  a0450020 v13 a<p>Hydrogels are considered as excellent candidates for tissue substitutes by virtue of their high water content and biphasic nature. However, the fact that they are soft, wet and floppy renders them difficult to process and use as custom-designed scaffolds. To address this problem alginate hydrogels were modeled and characterized by measuring stress-strain and creep behavior as well as viscosity as a function of sodium alginate concentration, cross-linking time and calcium ion concentration. The gels were then microfabricated into scaffolds using the pressure-assisted microsyringe. The mechanical and viscous characteristics were used to generate a processing window in the form of a phase diagram which describes the fidelity of the scaffolds as a function of the material and machine parameters. The approach can be applied to a variety of microfabrication methods and biomaterials in order to design well-controlled custom scaffolds.</p>
10aBioengineering1 aTirella, A1 aOrsini, A1 aVozzi, G.1 aAhluwalia, A.  uhttp://www.ncbi.nlm.nih.gov/pubmed/20811111